Part:BBa_J202039:Design

Ptet Characterization (Mutant#1)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 953
  Illegal NheI site found at 976
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 725
  

Design Notes

TetR(C0040) is expressed under constitutive promoter (J23101). TetR binds O2 sites in Ptet, and binding can be measured by GFP expression.

Source

Built from new part (J202031) and existing parts in the registry though BioBrick assembly.